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Updated: Dec 8, 2025

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
06:54

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

13.9K

Role of TG2 and TGF-β1 in the pathogenesis of human breast cancer

Hai-Cheng Gao1, Yan-Zhi Huang2, Yu-Qi Liu2

  • 1Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China.

Oncology Letters
|September 23, 2020
PubMed

Insights

Transforming growth factor-β1 (TGF-β1) and tissue transglutaminase (TG2) show elevated levels in breast cancer tissues. These factors play a synergistic role in MCF-7 breast cancer cells, influencing their expression and response to treatments.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Transforming growth factor-β1 (TGF-β1) and tissue transglutaminase (TG2) are implicated in cancer progression.
  • Understanding their interplay in breast cancer is crucial for therapeutic development.

Purpose of the Study:

  • To investigate the roles of TGF-β1 and TG2 in breast cancer.
  • To analyze their protein levels in MCF-7 cells under various conditions, including cisplatin treatment and modulation of their pathways.

Main Methods:

  • Immunohistochemical staining, immunofluorescence, and western blotting were employed.
  • Bioinformatics analysis was used to predict associations between TGF-β1 and TG2.
  • MCF-7 cells were treated with cisplatin, TGF-β1, and inhibitors of both factors.

Main Results:

  • Higher expression of TG2 and TGF-β1 was observed in breast cancer tissues compared to paracancerous tissues.
  • Cisplatin treatment reduced TG2 and TGF-β1 fluorescence intensity in MCF-7 cells.
  • Inhibition of TGF-β1 and TG2 decreased their expression, while TGF-β1 induction increased it, suggesting a synergistic interaction.

Conclusions:

  • TGF-β1 and TG2 play significant roles in breast cancer tissues and MCF-7 cells.
  • A synergistic relationship between TG2 and TGF-β1 was identified in MCF-7 cells, impacting their biological behavior.
Keywords:
breast cancercell deathcisplatintissue transaminasetransforming growth factor-β1

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