Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Complexation Properties of Self-Defensive Microgel-Modified Antimicrobial Surfaces.

ACS applied bio materials·2026

Same author

Expression profiles analysis and roles in immunity of transient receptor potential (TRP) channel genes in Spodoptera frugiperda.

BMC genomics·2025

Same author

Macroscale-area patterning of three-dimensional DNA-programmable frameworks.

Nature communications·2025

Same author

Controlling the roll-to-helix transformation in electron-beam-patterned gel-based micro-ribbons.

Soft matter·2024

Same author

Elasto-plastic effects on shape-shifting electron-beam-patterned gel-based micro-helices.

Materials horizons·2024

Same author

Distribution analysis of TRH in Bactrocera dorsalis using a CRISPR/Cas9-mediated reporter knock-in strain.

Insect molecular biology·2024

Same journal

A two-step centrifugal microfluidic platform for semi-automated IGRA detection of tuberculosis based on chemiluminescence.

The Analyst·2026

Same journal

On-site rapid identification of animal and plant creams <i>via</i> 2D FeB nanozyme-based colorimetric sensors.

The Analyst·2026

Same journal

Sensitive detection of aflatoxin B1 using a dual-mode fluorescent aptasensor based on cascade signal amplification.

The Analyst·2026

Same journal

Deep learning-enabled microfluidic digital PCR platform for efficient seven-color quantification.

The Analyst·2026

Same journal

Monitoring food spoilage biogenic amines utilizing a blue-emitting fluorescent ionic liquid.

The Analyst·2026

Same journal

Correction: Regeneration-on-a-chip: a planarian microfluidic device enabling automated cultivation, individual tracking and <i>in vivo</i> imaging for regeneration study.

The Analyst·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 8, 2025

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Integrating nucleic acid sequence-based amplification and microlensing for high-sensitivity self-reporting detection.

Feiyue Teng¹, Xinpei Wu, Tao Hong

¹Department of Chemical Engineering and Materials Science, Stevens Institute of Technology, Hoboken, New Jersey 07030, USA. mlibera@stevens.edu.

|September 23, 2020

Summary

This summary is machine-generated.

This study integrates microgels with molecular beacons, microlenses, and nucleic acid amplification for sensitive viral detection. The combined approach achieves ultra-low detection limits down to 10-18 M.

More Related Videos

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

Published on: October 7, 2025

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Related Experiment Videos

Last Updated: Dec 8, 2025

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

Published on: October 7, 2025

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Area of Science:

Biotechnology
Materials Science
Molecular Diagnostics

Background:

Developing sensitive and rapid viral detection methods is crucial for public health.
Microfluidic and microarray technologies offer platforms for integrated diagnostics.

Purpose of the Study:

To develop a novel microarray model for enhanced viral detection.
To integrate multiple functional elements for improved sensitivity.

Main Methods:

Utilized electron-beam patterned functional microgels.
Incorporated self-reporting molecular beacons and dielectric microlenses.
Integrated solid-phase and solution-phase nucleic acid amplification.

Main Results:

Achieved detection limits ranging from 10-10 M (direct hybridization) to 10-18 M (fully integrated system).
Demonstrated the synergistic effect of integrating multiple detection components.

Conclusions:

The developed microarray model significantly enhances viral detection sensitivity.
Electron-beam patterned microgels provide a versatile platform for integrating complex diagnostic functionalities.