Related Experiment Video
Updated: Dec 8, 2025
Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
Published on: March 17, 2023
A Localized surface plasmon resonance (LSPR) sensor integrated automated microfluidic system for multiplex
Jhih-Siang Chen1, Pin-Fan Chen, Hana Tzu-Han Lin
1Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei, 10617, Taiwan. nthuang@ntu.edu.tw.
This study presents a novel localized surface plasmon resonance (LSPR) biosensor integrated with an automated microfluidic system for rapid, simultaneous detection of inflammatory biomarkers. This technology enables efficient immune status monitoring and disease diagnosis with minimal sample volume.
Area of Science:
- Biomedical Engineering
- Biosensing Technology
- Immunodiagnostics
Background:
- Inflammation is a key indicator in diseases like cancer, cardiovascular, and neurological disorders.
- Measuring inflammatory biomarkers (e.g., immunoglobulins, cytokines, C-reactive protein) simultaneously is crucial due to their intercorrelation.
- Current methods for biomarker detection can be complex and time-consuming.
Purpose of the Study:
- To develop a label-free biosensing platform for continuous and dynamic inflammatory biomarker detection.
- To integrate localized surface plasmon resonance (LSPR) sensing with an automated microfluidic system.
- To simplify immunoassay reagent operations and reduce manual errors.
Main Methods:
- Utilized localized surface plasmon resonance (LSPR) for label-free molecule sensing.
- Developed an automated microfluidic control system for reagent guiding and switching.
- Performed multiplexed detection of IgG, TNF-α, and C-reactive protein (CRP).
Main Results:
- Demonstrated successful multiplexed measurement of IgG, TNF-α, and CRP.
- Achieved high sensitivity with a small assay volume (60 μL) and short assay time (3.5 h).
- Enabled 20 sensing spot measurements per test under four reagent conditions.
Conclusions:
- The integrated LSPR sensor and automated microfluidic system offers rapid, multiplexed, and multiparallel inflammatory biomarker detection.
- This technology has potential applications in immune status monitoring and the diagnosis/prognosis of inflammatory diseases.
- The system reduces manual operation complexity and potential errors in immunoassays.
