Magnetic sensor based on image processing for dynamically tracking magnetic moment of single magnetic mesenchymal
Haoyao Wang1, Yuqing Ge2, Jianfei Sun1
1State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, PR China.
Biosensors & Bioelectronics
|September 23, 2020
Summary
We developed an economical method to measure magnetic moments of mesenchymal stem cells (MSCs) labeled with superparamagnetic iron oxide (SPIO) nanoparticles. This technique accurately quantifies magnetic properties of single viable cells for improved cell tracking applications.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Nanotechnology
Background:
- Accurate measurement of magnetic moments in mesenchymal stem cells (MSCs) labeled with superparamagnetic iron oxide (SPIO) nanoparticles is essential for effective cell tracking.
- Existing micro/nanoscale magnetic moment measurement techniques have limitations, hindering precise analysis of single viable cells.
Purpose of the Study:
- To develop an economical and universal method for quantifying the magnetic moments of single viable SPIO-labeled MSCs.
- To establish a reliable system for evaluating the magnetic properties of individual cells for advanced cell tracking.
Main Methods:
- Utilized a gradient magnetic field generated by a nickel needle to track cell motion.
- Employed a simple, quantifiable magnetic sensor and an image-processing algorithm to measure magnetic moments of single MSCs.
- Validated the system by comparing measurements with a superconducting quantum interference device and inductively coupled plasma spectrometry.
Main Results:
- The proposed method demonstrated satisfactory agreement with established techniques (SQUID, ICP spectrometry), confirming its accuracy.
- Successfully measured magnetic moments of single viable MSCs labeled with SPIO nanoparticles.
- The system proved to be both economical and universal for micro/nanoscale magnetic moment measurements.
Conclusions:
- The developed miniature magnetic sensor system offers an accurate, economical, and universal solution for measuring magnetic moments of single SPIO-labeled MSCs.
- This provides a foundational method for SPIO-based labeling and tracking of MSCs, overcoming limitations of existing technologies.
- The system enables quantitative analysis of magnetic properties in single viable cells, advancing cell tracking applications.
