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An energy-dispersive X-ray fluorescence method for analyzing Fe and Zn in common bean, maize and cowpea
Georgia E Guild1, Nicholas G Paltridge1, Meike S Andersson2
1College of Science & Engineering, Flinders University, GPO Box 2100, Adelaide, South Australia 5001, Australia.
Background And Aims:
Biofortification breeding programs have the need for rapid and accurate screening methods to identify nutrient dense genotypes. This study explores the use of energy dispersive x-ray fluorescence (EDXRF) for the rapid screening of iron (Fe) and zinc (Zn) concentration in three coarse-grain crops; common bean, maize and cowpea.
Methods:
Bean, maize and cowpea seed was provided from biofortification breeding programs and analysed with Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) to determine reference Fe and Zn concentrations. A subset of samples for each crop was used to calibrate for Zn and Fe and a separate set of samples used to validate the XRF method.
Results:
Results indicate that when analysing bean, maize and cowpea flour samples, the EDXRF results were not significantly different to the reference ICP-MS analysis, with an average difference of ± 1 mg kg-1 for both Fe and Zn.
Conclusion:
EDXRF analysis of common beans, cowpea and maize flour enables rapid and accurate analysis when screening for Fe and Zn in bean, maize and cowpea.
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