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Quantitative analysis of a nuclear antigen in interphase and mitotic cells
Cytometry
|May 1, 1987
Summary
Researchers quantified p105, an interchromatin-associated antigen, during cell mitosis. A tenfold increase in p105 was observed from G2 to M phase, enabling mitotic index quantification.
Area of Science:
- Cell Biology
- Molecular Biology
- Immunology
Background:
- The interchromatin-associated antigen p105 plays a role in cellular processes.
- Understanding antigen dynamics during the cell cycle is crucial for cell biology research.
Purpose of the Study:
- To quantify the levels of p105 during cellular mitosis.
- To explore the utility of p105 as a marker for mitotic cells.
Main Methods:
- Indirect immunofluorescence microscopy
- Immunogold electron microscopy
- Multiparameter flow cytometry
- Quantitative immunoblot analysis
Main Results:
- A qualitative increase in p105 was observed in the mitotic cytoplasm.
- Flow cytometry revealed a tenfold increase in intracellular p105 from G2 to M phase.
- Quantitative immunoblot analysis corroborated the flow cytometry findings.
Conclusions:
- Increased p105 levels during mitosis can be effectively quantified using anti-p105 immunofluorescence and flow cytometry.
- p105 serves as a reliable marker for determining mitotic index and isolating mitotic cells.
- The study discusses the function and modulation of p105 throughout the cell cycle.