Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Inner Mitochondrial Membrane01:28

The Inner Mitochondrial Membrane

4.2K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria.  In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
4.2K
Mitochondrial Membranes01:45

Mitochondrial Membranes

15.7K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
15.7K
Mechanisms of Membrane-bending01:15

Mechanisms of Membrane-bending

3.1K
The living membranes are flexible due to their fluid mosaic nature; however, their bending into different shapes is an active process regulated by specific lipids and proteins. The membrane bending can be transient as seen in vesicles or stable for a long time as in microvilli. Cells regulate the size, location, and duration of the membrane curvature.
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
3.1K
Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

6.6K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
6.6K
Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

3.6K
Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with...
3.6K
Transport Across the Golgi01:26

Transport Across the Golgi

5.5K
While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Endothelial progenitor cell susceptibility to DNA damaging and DDR-modulating compounds determines endothelial differentiation accuracy.

Stem cell research & therapy·2026
Same author

Cell biology: Killing the prisoner escaping from mitochondria.

Current biology : CB·2026
Same author

CRISPR/Cpf1-mediated editing of PINK1 in induced pluripotent stem cells.

Stem cell research·2025
Same author

CRISPR/Cas9-mediated editing of MIC13 in human induced pluripotent stem cells: A model for mitochondrial hepato-encephalopathy.

Stem cell research·2025
Same author

Ubiquitin precursor with C-terminal extension promotes proteostasis and longevity.

Molecular cell·2025
Same author

Controlling mitochondrial membrane architecture via MIC60 determines viral replication to promote anti-viral immunity.

Cell reports·2025
Same journal

Horizontal transfer of mitochondria in cancer: The physiology reborn in disease?

Trends in cell biology·2026
Same journal

Spindle errors: A stress test for epithelial robustness.

Trends in cell biology·2026
Same journal

Multicellular ecosystems: Linking cellular diversity to tissue function and disease.

Trends in cell biology·2026
Same journal

Orchestrating the signaling-bias at the protease-activated receptor, PAR1.

Trends in cell biology·2026
Same journal

Crashing by design: Utilizing DNA damage for MCC differentiation.

Trends in cell biology·2026
Same journal

The value of a shared lab: Our insights.

Trends in cell biology·2026
See all related articles

Related Experiment Video

Updated: Dec 7, 2025

Author Spotlight: Decoding Mitochondrial Aging
08:48

Author Spotlight: Decoding Mitochondrial Aging

Published on: June 30, 2023

4.6K

Cristae Membrane Dynamics - A Paradigm Change.

Arun Kumar Kondadi1, Ruchika Anand1, Andreas S Reichert1

  • 1Institute of Biochemistry and Molecular Biology I, Medical Faculty, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany.

Trends in Cell Biology
|September 26, 2020
PubMed
Summary
This summary is machine-generated.

Mitochondrial cristae, once thought static, are now known to be dynamic bioenergetic units. Recent super-resolution microscopy reveals rapid remodeling, changing our fundamental understanding of mitochondria.

Keywords:
MICOSOPA1cristae dynamicsremodeling

More Related Videos

A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics
10:31

A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics

Published on: September 2, 2020

7.9K
Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy
08:55

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy

Published on: February 17, 2023

3.8K

Related Experiment Videos

Last Updated: Dec 7, 2025

Author Spotlight: Decoding Mitochondrial Aging
08:48

Author Spotlight: Decoding Mitochondrial Aging

Published on: June 30, 2023

4.6K
A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics
10:31

A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics

Published on: September 2, 2020

7.9K
Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy
08:55

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy

Published on: February 17, 2023

3.8K

Area of Science:

  • Cell Biology
  • Mitochondrial Biology
  • Bioenergetics

Background:

  • Mitochondria are vital organelles with diverse metabolic roles.
  • Cristae, mitochondrial inner membrane infoldings, exhibit varied architecture.
  • Previously, cristae were considered static structures.

Purpose of the Study:

  • To summarize recent findings on mitochondrial cristae dynamics.
  • To discuss the functional implications of cristae remodeling.
  • To highlight the role of MICOS and OPA1 in cristae organization.

Main Methods:

  • Advanced super-resolution microscopy techniques.
  • Mechanistic studies of molecular players.
  • Structural analysis of mitochondrial components.

Main Results:

  • Mitochondrial cristae are highly dynamic, independent bioenergetic units.
  • Cristae remodel on a timescale of seconds.
  • Key proteins like MICOS and OPA1 are crucial for cristae structure and dynamics.

Conclusions:

  • Mitochondrial cristae are dynamic and actively remodel.
  • These dynamics are essential for mitochondrial function and regulation.
  • New insights fundamentally alter our view of mitochondrial architecture and bioenergetics.