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Generation and Characterization of Mouse Models for Skeletal Disease
Gabrielle E Foxa1, Ye Liu1, Lisa M Turner2
1Program for Skeletal Disease and Tumor Microenvironment, Center for Cancer and Cell Biology, Van Andel Institute, Grand Rapids, MI, USA.
Methods in Molecular Biology (Clifton, N.J.)
|September 26, 2020
Summary
Genetically engineered mouse models (GEMMs) help study skeletal diseases like osteoarthritis. Protocols for inducing experimental osteoarthritis and analyzing bone structure using micro-CT and histomorphometry are detailed.
Area of Science:
- Biomedical Engineering
- Genetics
- Orthopedics
Background:
- Genetically engineered mouse models (GEMMs) are crucial for understanding skeletal diseases.
- Osteoarthritis (OA) research often utilizes GEMMs to investigate genetic factors.
- Surgical induction of OA in mice models human disease progression.
Purpose of the Study:
- To detail protocols for inducing experimental osteoarthritis in mice.
- To describe methods for analyzing skeletal phenotypes in GEMMs.
- To provide a framework for studying genetic contributions to OA.
Main Methods:
- Induction of experimental osteoarthritis via surgical methods in mice.
- Analysis of skeletal phenotypes using micro-computerized tomography (micro-CT).
- Skeletal histomorphometry for detailed bone structure assessment.
Main Results:
- Established protocols for reproducible OA induction in GEMMs.
- Demonstrated efficacy of micro-CT and histomorphometry for skeletal analysis.
- Provided quantitative data on OA-related skeletal changes in mice.
Conclusions:
- GEMMs are valuable tools for investigating OA pathogenesis.
- Detailed protocols enhance reproducibility in OA research.
- Advanced imaging and histological techniques facilitate comprehensive skeletal phenotyping.
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