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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

842
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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Spectrochemical Probing of MicroRNA Duplex Using Spontaneous Raman Spectroscopy for Biosensing Applications.

Preetham Ravi1,2,3,4, Surya Pratap Singh5,6, Jeon Woong Kang5

  • 1Center for Engineered Cancer Testbeds, and Department of Civil and Environmental Engineering, North Dakota State University, Fargo, North Dakota 58108, United States.

Analytical Chemistry
|September 28, 2020
PubMed
Summary
This summary is machine-generated.

Raman spectroscopy detects structural differences in microRNAs (miRNAs) and their hybrids. This technique can identify nucleic acid backbone variations, aiding in miRNA diagnostics and therapeutics.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Spectroscopy

Background:

  • MicroRNAs (miRNAs) are crucial in human diseases, necessitating precise structural understanding for diagnostics and therapeutics.
  • Modifications in synthetic oligonucleotides require accurate structural analysis to develop effective diagnostic tools and therapeutic agents.

Purpose of the Study:

  • To investigate structural variations in sense and antisense microRNA-21 using label-free spontaneous Raman spectroscopy.
  • To explore the utility of Raman spectroscopy in differentiating nucleic acid structures and interactions.

Main Methods:

  • Utilized label-free spontaneous Raman spectroscopy.
  • Analyzed structural differences in microRNA-21 by hybridizing with complementary RNA and DNA oligonucleotides.

Main Results:

  • Observed distinct changes in the Raman band at 785 cm-1, attributed to the phosphodiester bond in the nucleic acid backbone.
  • Demonstrated that this Raman band can serve as a marker for identifying structural variations in nucleic acids.
  • Supported the application of Raman spectroscopy in distinguishing intramolecular (single-stranded RNA/DNA) and intermolecular (hybridized nucleic acids) interactions.

Conclusions:

  • Raman spectroscopy is effective in discerning structural differences in nucleic acids, including microRNAs.
  • This technique holds potential for developing sensitive biosensors for quantifying microRNAs in clinical settings.
  • Findings can inform the design of more robust and functional therapeutic microRNAs.