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Updated: Dec 7, 2025

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis
Published on: May 10, 2020
The undefined N source might be overestimated by 15 N tracer trials
Zhi Quan1,2,3,4, Xin Zhang2, Yunting Fang1,4,5
1Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China.
Abstract:
Based on data from previous 15 N-tracer trials, Yan et al. (Global Change Biology, 26, 191-199; 2020) accounted the sources of cereal crop nitrogen (N) from fertilizer inputs (from both current and previous seasons) and possible uptake of nonfertilizer N inputs, leaving a significant part of N source undefined. We argue that the undefined N source does not reflect the net change in soil N stock and might be overestimated for three reasons: (a) higher yield in 15 N tracer trials; (b) a low residue return rate is assumed; (c) underestimated contribution by fertilizer inputs from previous seasons due to the lack of long-term trials.
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