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Thermometric enzyme linked immunosorbent assay: TELISA.
Biochimica Et Biophysica Acta
|August 11, 1977
Summary
A novel thermometric enzyme linked immunosorbent assay (TELISA) enables sensitive detection of compounds in biological fluids. This method measures enzymic heat formation for precise quantification, achieving high sensitivity in model systems.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Immunology
Background:
- Enzyme linked immunosorbent assay (ELISA) is a widely used technique for detecting and quantifying substances.
- Measuring heat generated by enzymatic reactions offers a sensitive detection principle.
Purpose of the Study:
- To describe a new assay method, thermometric enzyme linked immunosorbent assay (TELISA).
- To evaluate the applicability of TELISA for quantifying compounds in biological fluids.
Main Methods:
- Developed TELISA by integrating ELISA with an enzyme thermistor.
- Used rabbit antihuman serum albumin immobilized on Sepharose CL-4B as the immunosorbent.
- Employed both normal and catalase-labeled human serum albumin in a competitive binding assay.
Main Results:
- Successfully determined human serum albumin concentrations as low as 10(-10) M (5 ng/ml).
- Demonstrated the feasibility of using heat measurement for sensitive immunoassays.
Conclusions:
- TELISA is a sensitive and viable method for assaying biological compounds.
- The integration of thermometry with ELISA enhances detection capabilities for biological fluid analysis.