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Advances and challenges for fluorescence nanothermometry.

Jiajia Zhou1, Blanca Del Rosal2, Daniel Jaque3,4

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Fluorescent nanothermometers offer advanced temperature sensing in biological systems. However, potential biased sensing requires careful consideration for accurate cellular and in vivo measurements.

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Optical Imaging

Background:

  • Fluorescent nanothermometers are crucial for monitoring temperature in biological systems.
  • Advancements have led to improved resolution and multifunctionality in nanothermometry.
  • Concerns exist regarding potential biased sensing in fluorescence-based detection methods.

Purpose of the Study:

  • To review design principles and recent advances in fluorescence nanothermometry.
  • To highlight key application achievements in biosensing.
  • To discuss challenges, including biased sensing, and propose future directions.

Main Methods:

  • Review of design principles for temperature-responsive materials.
  • Analysis of detection procedures for high-resolution temperature mapping.
  • Discussion of potential sources of error and bias in fluorescence measurements.

Main Results:

  • Recent nanothermometers demonstrate enhanced performance and multifunctionality.
  • Applications include monitoring intracellular organelle and internal organ metabolism.
  • Potential for biased sensing has been identified in fluorescence-based detection.

Conclusions:

  • Fluorescence nanothermometry is a rapidly advancing field with significant biological applications.
  • Addressing biased sensing is critical for reliable temperature measurements.
  • Future research should focus on fundamental improvements and practical implementation challenges.