Related Experiment Video
Updated: Dec 7, 2025
Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
Published on: April 22, 2019
SNHG3/miR-2682-5p/HOXB8 promotes cell proliferation and migration in oral squamous cell carcinoma
1Department of Stomatology, North China University of Science and Technology Affiliated Hospital, Tangshan, China.
Objective:
The potential molecular mechanism underlying the disease progression of oral squamous cell carcinoma (OSCC) remains largely elusive. The purpose of the study is to figure out the role and molecular mechanism of homeobox B8 (HOXB8) in OSCC.
Materials And Methods:
The expression level of HOXB8 in OSCC was validated by RT-qPCR. The functions of HOXB8 in OSCC cells were identified through loss-of-function assays, including CCK-8 assay, colony formation assay, transwell assay, and immunofluorescence (IF). The upstream miRNA that could directly target HOXB8 was searched out through bioinformatics analysis and luciferase reporter assay. Mechanism experiments were further conducted to predict the long non-coding RNA (lncRNA) that could positively regulate HOXB8 and compete for miR-2682-5p with HOXB8.
Results:
HOXB8 was markedly upregulated in OSCC tissues and cell lines. Furthermore, cell proliferation and migration were inhibited due to the shortage of HOXB8. HOXB8 was targeted by miR-2682-5p that negatively regulated cell proliferation and migration. Small nucleolar RNA host gene 3 (SNHG3) acted as a sponge for miR-2682-5p. Inhibition of miR-2682-5p or the overexpression of HOXB8 rescued the effects of SNHG3 silencing on the proliferation and migration.
Conclusion:
HOXB8 is regulated by SNHG3/miR-2682-5p axis to promote OSCC cell proliferation and migration.
Related Concept Videos
02:23Abnormal Proliferation
02:33Hedgehog Signaling Pathway
02:38Mitogens and the Cell Cycle
01:24Small GTPases - Ras and Rho
Three regulatory proteins control their activity:
01:20The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
02:53Adaptive Mechanisms in Cancer Cells
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...