Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

X-ray Crystallography

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...

$X-ray Diffraction of Biological Samples$ 01:10

X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Impact of Early Initiation of Automated Insulin Delivery on Glycemia Two Years from Type 1 Diabetes Diagnosis.

Hormone research in paediatrics·2026

Same author

Programmable DNA Folding Modulates Phase Behavior and Dynamics of DNA/Peptide Condensates.

ACS nano·2026

Same author

Single cell transcriptomics reveals enrichment of aggregation-prone alpha-synuclein isoforms across synucleinopathies.

bioRxiv : the preprint server for biology·2025

Same author

Structural basis for ATP-driven double-ring assembly of the human mitochondrial Hsp60 chaperonin.

bioRxiv : the preprint server for biology·2025

Same author

Correction to "Self-Assembly of Accumulated Sphingolipids into Cytotoxic Fibrils in Globoid Cell Leukodystrophy and Their Inhibition by Small Molecules in Vitro".

ACS nano·2025

Same author

A Common Lines Approach for Ab Initio Modeling of Molecules with Tetrahedral and Octahedral Symmetry.

SIAM journal on imaging sciences·2025

Same journal

Correction: A method for supervoxel-wise association studies of age and other non-imaging variables from coronary computed tomography angiograms.

Scientific reports·2026

Same journal

Poly(bromophenol blue)/CoSn(OH)<sub>6</sub> cubic particles modified pencil graphite electrode for electrochemical determination of diphenhydramine.

Scientific reports·2026

Same journal

Dietary Chlorella, Spirulina, and acidifier modulate jejunal cytokine-related gene expression in broiler chickens.

Scientific reports·2026

Same journal

Perceived physical activity barriers in university students: associations with fatigue and eating behaviours.

Scientific reports·2026

Same journal

Refuge limitation structures habitat use in agricultural landscapes: evidence from Sunda pangolins.

Scientific reports·2026

Same journal

Lightweight stateless transaction verification with outsourced witness updates for UTXO blockchains.

Scientific reports·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 7, 2025

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

Angular super-resolution retrieval in small-angle X-ray scattering.

Benjamin Gutman^1,2, Michael Mrejen^3,2, Gil Shabat¹

¹School of Mathematical Sciences, Tel Aviv University, 69978, Tel Aviv, Israel.

Scientific Reports

|September 30, 2020

Summary

This summary is machine-generated.

This study introduces a novel two-step method to significantly improve the angular resolution of small-angle X-ray scattering (SAXS) techniques. The enhanced resolution allows for more detailed nanoscopic structure analysis in various experimental settings.

More Related Videos

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution

Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution

Published on: January 8, 2013

Related Experiment Videos

Last Updated: Dec 7, 2025

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution

Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution

Published on: January 8, 2013

Area of Science:

Materials Science
Nanotechnology
Analytical Chemistry

Background:

Small-angle X-ray scattering (SAXS) is crucial for nanoscopic characterization.
Laboratory-based SAXS systems face limitations in X-ray flux and angular resolution compared to synchrotron sources.

Purpose of the Study:

To develop a methodology for enhancing angular resolution in SAXS.
To enable finer nanoscopic structure analysis with existing SAXS apparatuses.

Main Methods:

A two-step retrieval methodology was developed.
Minute hardware additions included detector translation in subpixel steps.
Modification of the incoming beam shape was employed to generate a set of 2D scattering images.

Main Results:

The developed technique successfully enhanced angular resolution for SAXS.
Experimental verification confirmed superior resolution capabilities.
The method demonstrated the ability to resolve finer nanoscopic structures.

Conclusions:

The proposed super-resolution SAXS retrieval technique significantly improves resolution.
This advancement can be implemented in most existing SAXS setups, both lab- and synchrotron-based.
The method offers a practical approach to advanced nanoscopic characterization.