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Related Experiment Video

Updated: Dec 7, 2025

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

Published on: June 19, 2013

Rapid Lentiviral Vector Producer Cell Line Generation Using a Single DNA Construct.

Yu Hua Chen¹, Celeste Pallant¹, Christopher J Sampson¹

¹GlaxoSmithKline Medicines Research Centre, Gunnels Wood Road, Stevenage SG1 2NY, UK.

Molecular Therapy. Methods & Clinical Development

|September 30, 2020

Summary

This summary is machine-generated.

Stable producer cell lines for lentiviral vector production offer an alternative to transient transfection. This method improves scalability and yields, potentially increasing patient access to lentiviral vector-based medicines.

Keywords:

cell line development lentiviral vector manufacturing producer cell line

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Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

Published on: May 24, 2012

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Last Updated: Dec 7, 2025

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

Published on: June 19, 2013

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

Published on: May 24, 2012

Area of Science:

Biotechnology
Molecular Biology
Gene Therapy

Background:

Current lentiviral vector production relies on transient transfection of adherent cells, limiting scalability.
Vesicular stomatitis virus envelope glycoprotein (VSVg)-pseudotyped lentiviral vectors are crucial for gene therapy applications.

Purpose of the Study:

To develop a method for rapidly generating stable suspension producer cell lines for lentiviral vectors.
To improve the efficiency and scalability of lentiviral vector manufacturing.

Main Methods:

Stable transfection of 293T cells with a single DNA construct encoding all lentiviral vector components.
Generation of suspension cell lines for large-scale production.

Main Results:

Achieved titers comparable to transient transfection methods.
Demonstrated genetic and functional stability in extended cell culture.
Facilitated scalability in single-use stirred-tank bioreactors.

Conclusions:

Stable suspension producer cell lines offer a scalable and efficient alternative for lentiviral vector production.
This approach can significantly increase batch yields and improve patient access to lentiviral vector-based therapies.