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Updated: Dec 7, 2025

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
Published on: June 24, 2013
Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron
Lucio Ayres Caldas1,2,3, Fabiana Avila Carneiro4, Luiza Mendonça Higa5
1Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Cidade Universitária. Av., Carlos Chagas Filho 373, Prédio CCS, Bloco C, subsolo, CEP: 21941902, Rio de Janeiro, RJ, Brazil. lucio@biof.ufrj.br.
Abstract:
SARS-CoV-2 is the cause of the ongoing COVID-19 pandemic. Here, we investigated the interaction of this new coronavirus with Vero cells using high resolution scanning electron microscopy. Surface morphology, the interior of infected cells and the distribution of viral particles in both environments were observed 2 and 48 h after infection. We showed areas of viral processing, details of vacuole contents, and viral interactions with the cell surface. Intercellular connections were also approached, and viral particles were adhered to these extensions suggesting direct cell-to-cell transmission of SARS-CoV-2.
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