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Related Experiment Video

Updated: Dec 7, 2025

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The crosstalk between platelets and body fat: A reverse translational study.

Shuang Han1, Peng Wu1, Meng Duan2

  • 1Chronic Disease Research Institute, The Children's Hospital, National Clinical Research Center for Child Health, School of Public Health, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China; Department of Nutrition and Food Hygiene, School of Public Health, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China; School of Basic Medical Sciences, Zhejiang University, Hangzhou, Zhejiang, China.

Clinical Nutrition (Edinburgh, Scotland)
|October 3, 2020
PubMed
Summary

Platelets significantly increase pre-adipocyte proliferation, potentially explaining the link between higher platelet counts and increased body fat. This study reveals platelets

Keywords:
Cyclin D1PlateletPre-adipocyte proliferationmTOR

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Area of Science:

  • Cell Biology
  • Hematology
  • Metabolic Research

Background:

  • Previous population studies show a positive association between platelet counts and body fat percentage in humans.
  • Obesity is linked to altered platelet counts and function, suggesting a role in metabolic dysregulation.

Purpose of the Study:

  • To investigate the role of platelets in modulating pre-adipocyte proliferation using a reverse translational approach.
  • To elucidate the molecular mechanisms by which platelets influence adipogenesis.

Main Methods:

  • Co-culture of mouse (3T3-L1) and human pre-adipocytes with isolated platelets or platelet releasate.
  • Assessment of cell viability and proliferation using MTT and flow cytometry assays.
  • Western blotting to analyze protein phosphorylation in the mTOR pathway.

Main Results:

  • Increased platelet counts were observed in adipose tissue of obese mice compared to lean controls.
  • Platelets and activated platelet releasate significantly stimulated pre-adipocyte proliferation in vitro.
  • Platelets from obese mice showed enhanced proliferative effects on pre-adipocytes.
  • Platelet-induced proliferation involved acceleration of cell cycle progression (G0/G1 to S phase) via mTOR pathway activation and cyclin D1 upregulation.

Conclusions:

  • Platelets and their releasates are key regulators of pre-adipocyte proliferation.
  • This study provides mechanistic insights into the causal link between platelets and body fat accumulation.