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Updated: Dec 7, 2025

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
Published on: February 16, 2017
Calorie restriction changes muscle satellite cell proliferation in a manner independent of metabolic modulation
Phablo Abreu1, Julian D C Serna1, Ana C Munhoz1
1Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.
Abstract:
Calorie restriction is known to promote healthy aging, which includes prevention of muscle loss. We investigated the effect of rodent calorie restriction on mitochondrial respiration and clonogenic capacity of muscle satellite stem cells, since metabolic alterations are known to regulate stem cell activity. Surprisingly, short or long-term calorie restriction do not change mitochondrial or glycolytic function. Nevertheless, both short- and long-term calorie restriction enhance myogenic colony formation. Overall, our results show that not all changes in satellite stem cell function are accompanied by metabolic remodeling.
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