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Optical fiber bio-sensor for phospholipase using liquid crystal.

Jieyuan Tang1, Zhibin Li1, Mengyuan Xie1

  • 1Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes, Jinan University, Guangzhou, 510632, China; Department of Optoelectronic Engineering, Jinan University, Guangzhou, 510632, China.

Biosensors & Bioelectronics
|October 3, 2020
PubMed
Summary

A novel optical fiber sensor detects phospholipase A2 (PLA2) using a label-free method. This cost-effective sensor achieves a low detection limit (<1 nM) with high specificity and repeatability.

Keywords:
BiosensorFiberLiquid crystalPhospholipase A2Side-polished fiber

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Area of Science:

  • Biomedical Engineering
  • Chemical Sensors
  • Biotechnology

Background:

  • Phospholipase A2 (PLA2) is a crucial enzyme involved in various physiological and pathological processes.
  • Accurate and sensitive detection of PLA2 is essential for disease diagnosis and drug development.
  • Existing detection methods often lack sensitivity, specificity, or require complex procedures.

Purpose of the Study:

  • To develop a cost-effective and label-free optical fiber sensor for sensitive phospholipase A2 (PLA2) detection.
  • To investigate the sensor's performance in terms of detection limit, response time, specificity, and repeatability.
  • To demonstrate the potential of the sensor for real-time and online quantitative detection of biological molecules.

Main Methods:

  • Fabrication of an alkoxysilane-modified side-polished fiber (SPF) coated with 5CB and L-DLPC.
  • Utilizing the change in relative transmission optical power (RTOP) as an indicator of PLA2 activity.
  • Correlating the 5 dB RTOP variation response time with PLA2 concentration for quantitative analysis.

Main Results:

  • The optical fiber sensor successfully detected PLA2 in nM concentrations with a detection limit below 1 nM.
  • The sensor exhibited excellent specificity, with a significantly higher response to PLA2 compared to other enzymes.
  • The response-time showed an exponential dependence on PLA2 concentration, enabling rapid detection.

Conclusions:

  • The proposed label-free optical fiber sensor offers a sensitive, specific, and cost-effective method for PLA2 detection.
  • The sensor's ability to reduce detection time and its potential for integration into micro-flow chips are significant advantages.
  • This technology holds promise for real-time, online quantitative analysis of biological molecules in various applications.