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Published on: June 16, 2019
Proteomics identification of differential S-nitrosylated proteins between the beef with intermediate and high
Qiongniu Zhu1, Lujuan Xing1, Qin Hou1
1Key Laboratory of Meat Processing and Quality Control, Ministry of Education China, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Abstract:
This study aimed to identify the differential S-nitrosylated proteins at post-mortem in beef longissimus thoracis (LT) muscles from carcasses with intermediate (5.40 < pH < 5.80, n = 6) and high (pH ≥ 6.00, n = 6) ultimate pH (pHu). LT muscles were labeled with iodo-tandem mass tags (iodoTMT126-129). A total of 856 S-nitrosylated sites from 257 proteins were identified in high pHu beef LT muscles. The S-nitrosylated protein intensity in high pHu beef was higher compared to that of intermediate pHu beef along with a large number of cysteine sites. The motif revealed that the cysteine modifications played an essential role in cellular signaling and homeostasis. In high pHu beef, more up-regulated proteins were related to energy metabolism enzymes and mitochondrial dysfunction enzymes compared to down-regulated proteins which are involved in calcium homeostasis.
