Related Experiment Video

Updated: Dec 6, 2025

Author Spotlight: Modeling an Aspect of Preeclampsia in Female Mice Using Hypoxic Human Placenta-Derived Small Extracellular Vesicles
05:31

Author Spotlight: Modeling an Aspect of Preeclampsia in Female Mice Using Hypoxic Human Placenta-Derived Small Extracellular Vesicles

Published on: January 26, 2024

1.1K

IL-27 variants might be genetic risk factors for preeclampsia: based on genetic polymorphisms, haplotypes and in

Danial Jahantigh1, Saeedeh Ghazaey Zidanloo2, Forough Forghani3,4

  • 1Department of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran.

Molecular Biology Reports
|October 4, 2020
PubMed

Insights

Genetic variations in IL-27 (rs153109 and rs17855750) are linked to pre-eclampsia (PE) susceptibility. Specific genotypes and haplotypes increase PE risk, suggesting their potential as predictive indicators for this pregnancy disorder.

Area of Science:

  • Genetics
  • Obstetrics
  • Immunology

Background:

  • Pre-eclampsia (PE) is a pregnancy-specific disorder linked to adverse neonatal outcomes.
  • Overexpression of IL-27 and its receptor is frequently observed in trophoblast cells of PE patients.
  • Understanding the genetic basis of PE is crucial for identifying at-risk individuals.

Purpose of the Study:

  • To investigate the association between IL-27 gene polymorphisms (rs153109 and rs17855750) and pre-eclampsia in an Iranian population.
  • To evaluate the role of these genetic variations in PE susceptibility and severity.

Main Methods:

  • Case-control study involving 170 PE patients and 170 healthy pregnant women.
  • Genotyping of IL-27 rs153109 and rs17855750 polymorphisms using the PCR-RFLP method.
  • In-silico analysis to predict the functional impact of identified single nucleotide polymorphisms (SNPs).

Main Results:

  • Significantly higher frequencies of heterozygous and mutant homozygous genotypes for rs153109 were found in PE patients, particularly in severe and mild cases.
  • The rs17855750 gene polymorphism showed associations with PE susceptibility across total, severe, and early-onset subgroups.
  • Mutant GG haplotype (rs153109/rs17855750) increased PE risk, while wild AT haplotypes were protective.

Conclusions:

  • IL-27 gene polymorphisms rs153109 and rs17855750 are associated with genetic susceptibility to pre-eclampsia.
  • These SNPs may serve as potential biomarkers for predicting PE risk.
  • Functional in-silico analysis suggests that these polymorphisms can alter IL-27 gene expression and protein function.

Keywords:
Genetic polymorphismIL-27In-silicoPost translational modificationPreeclampsia

More Related Videos

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.5K
Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq09:52

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Published on: April 19, 2013

24.7K

Related Concept Videos

Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
17.6K
Genome-wide Association Studies-GWAS01:11

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
15.0K
Human Genetics01:28

Human Genetics

Human genetics provides a profound framework for understanding the interplay between genetic predispositions and human psychology. At the heart of this discipline lies the study of how genes influence physical traits, behaviors, and susceptibility to diseases. Each person carries a unique genetic code that subtly or significantly shapes their psychological and behavioral landscape.
The complex relationship between genetics and psychology is observable through common biological components such...
1.2K