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Enhancing sensitivity of lateral flow assay with application to SARS-CoV-2
Tao Peng1, Xiangpei Liu, L Garry Adams1
1Texas A&M University, College Station, Texas 77843, USA.
Applied Physics Letters
|October 5, 2020
Summary
Photon counting enhances lateral flow assays (LFAs) for sensitive biomarker detection. This photon-counting approach accurately quantifies SARS-CoV-2 antibodies, overcoming LFA limitations.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Immunodiagnostics
Background:
- Lateral flow assays (LFAs) are widely used for rapid biomarker detection due to their low cost and portability.
- Current LFAs suffer from qualitative readouts and limited sensitivity, hindering precise quantification.
- Overcoming these limitations is crucial for advancing diagnostic capabilities.
Purpose of the Study:
- To develop a photon-counting method for accurate quantification of LFAs.
- To enhance the sensitivity of LFA-based biomarker detection.
- To demonstrate the application of this technique for quantifying SARS-CoV-2 antibodies.
Main Methods:
- Implementation of a photon-counting optical analysis system.
- Adaptation of the system for analyzing lateral flow assay test lines.
- Quantification of antibody density on LFAs using laser-based optical detection.
Main Results:
- The photon-counting approach enabled accurate quantification of LFAs.
- Enhanced sensitivity was achieved compared to traditional LFA readout methods.
- Demonstrated successful quantification of SARS-CoV-2 antibody density.
Conclusions:
- Photon counting offers a sensitive and accurate method for quantifying LFAs.
- This technique significantly improves upon the limitations of conventional LFAs.
- The developed method shows promise for precise diagnostics, including for SARS-CoV-2 detection.
