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Updated: Dec 6, 2025
Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors
Published on: January 29, 2019
A potential approach for assessing the quality of human and nonhuman adenoviral vector preparations
Ekramy E Sayedahmed1, Suresh K Mittal1
1Department of Comparative Pathobiology, Purdue Institute for Immunology, Inflammation and Infectious Disease and Purdue University Center for Cancer Research, College of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907, USA.
Abstract:
Various types of human and nonhuman adenoviral (AdV) vectors are being used as gene delivery vectors in preclinical and clinical investigations. The objective of this study was to determine the ratio between the 2 best assays that would effectively address the variability in the titration of various AdV vectors in different cell lines and help obtain consistent results in preclinical and clinical studies using different AdV vectors. Here, we compared plaque-forming units, tissue culture infectious dose 50, focus-forming units (FFU), virus particle (VP) count, and genome copy number (GCN) of purified preparations of human AdV type C5, bovine AdV type 3, and porcine AdV type 3 to determine a correlation between infectious and noninfectious virus particles. Our results suggest that a VP:FFU or a VP:GCN ratio could accurately reflect the quality of an AdV preparation and could serve as an indicator to control batch-to-batch variability.
