Assessment of liver function reserve by photoacoustic tomography: a feasibility study

Assessment of liver function reserve (LFR) is essential to determine liver resection scope and predict prognosis for patients with liver disease. Indocyanine green (ICG) concentration change is a classic marker to reflect liver function reserve as ICG is selectively taken up and eliminated by liver. Here we proposed a noninvasive approach for LFR assessment based on a real-time photoacoustic tomography (PAT) system. This feasibility study was to detect ICG concentration change by PAT in phantom and in vivo using both normal and partial hepatectomy (PH) rabbits. A linear relationship between photoacoustic signal intensity of ICG and ICG concentration was found in vitro. In vivo ICG concentration change over time after ICG injection was observed by PAT in normal rabbits, which was consistent with the findings measured by invasive spectrophotometry. Finally, clear difference in ICG clearance between the control and PH models was identified by PAT. Taken together, our study indicated the clinical potential of PAT to in vivo evaluate LFR noninvasively.