Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.0K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.4K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.4K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.7K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.7K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.5K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.6K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.6K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

913
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
913

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Better reporting is better science: Community-defined minimal reporting requirements for light microscopy.

The Journal of cell biology·2026
Same author

A blind reconstruction approach for multifocal structured illumination microscopy.

Methods and applications in fluorescence·2025
Same author

Unified joint reconstruction approach for random illumination microscopy: retraction.

Biomedical optics express·2021
See all related articles

Related Experiment Video

Updated: Dec 6, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.8K

Unified joint reconstruction approach for random illumination microscopy.

Penghuan Liu1,2

  • 1College of Optical and Electronic Technology, China Jiliang University, 310018 Hangzhou, China.

Biomedical Optics Express
|October 5, 2020
PubMed
Summary

Random illumination microscopy (RIM) offers super-resolution imaging beyond the diffraction limit. This study introduces a unified reconstruction method for RIM, enhancing performance and simplifying hyperparameter tuning for better imaging quality.

More Related Videos

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

7.2K
Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
14:23

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

11.2K

Related Experiment Videos

Last Updated: Dec 6, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.8K
High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

7.2K
Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
14:23

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

11.2K

Area of Science:

  • Optical microscopy
  • Super-resolution imaging techniques
  • Image reconstruction algorithms

Background:

  • Random illumination microscopy (RIM) utilizes uncontrolled speckle patterns to overcome the Abbe diffraction limit.
  • Existing joint reconstruction methods for RIM have limitations in performance evaluation and hyperparameter tuning.
  • Conventional structured illumination microscopy (SIM) suffers from out-of-focus light degradation.

Purpose of the Study:

  • To review state-of-the-art joint reconstruction methods in RIM.
  • To propose a unified joint reconstruction approach for evaluating various regularization terms.
  • To address challenges like out-of-focus light degradation in SIM using RIM.

Main Methods:

  • A comprehensive review of current RIM joint reconstruction techniques.
  • Development of a unified reconstruction model for systematic evaluation of regularization terms.
  • Implementation and testing of the proposed unified approach with different priors, including the ℓ2,1 regularizer.

Main Results:

  • The unified model allows for robust and easy hyperparameter tuning.
  • The ℓ2,1 regularizer demonstrates effectiveness as a prior in practical RIM scenarios.
  • The proposed RIM setup inherently solves the out-of-focus light degradation issue seen in conventional SIM.

Conclusions:

  • The unified joint reconstruction approach provides a robust framework for RIM.
  • RIM, particularly with the proposed method, offers a versatile and cost-effective alternative to conventional super-resolution techniques.
  • This work advances RIM capabilities for improved biological and material science imaging.