Related Experiment Video
Updated: Dec 6, 2025
Light-driven Molecular Motors on Surfaces for Single Molecular Imaging
Published on: March 13, 2019
Janus Micromotors Coated with 2D Nanomaterials as Dynamic Interfaces for (Bio)-Sensing
Kaisong Yuan1,2, Miguel Ángel López1,3, Beatriz Jurado-Sánchez1,3
1Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcala, Alcala de Henares, E-28871 Madrid, Spain.
Janus micromotors with graphdiyne oxide, graphene oxide, or black phosphorous surfaces efficiently load and release peptides for biosensing. Surface properties and micromotor movement critically influence peptide sorption and release kinetics, enabling sensitive detection in complex samples.
Area of Science:
- Nanotechnology
- Biomaterials Science
- Analytical Chemistry
Background:
- Understanding nanomaterial-surface interactions is crucial for developing advanced biosensors.
- Janus micromotors offer unique propulsion and surface functionalization capabilities for targeted applications.
- Existing biosensing methods face challenges in complex biological samples, necessitating novel approaches.
Purpose of the Study:
- To investigate the sorption and desorption kinetics of affinity peptides on graphdiyne oxide (GDYO)-, graphene oxide (GO)-, and black phosphorous (BP)-wrapped Janus micromotors.
- To evaluate the influence of nanomaterial surface properties and micromotor movement on peptide loading/release.
- To demonstrate the feasibility of this micromotor-based system for detecting Cholera Toxin B in realistic biological environments.
Main Methods:
- Fabrication of Janus micromotors functionalized with GDYO, GO, and BP.
- Utilizing a fluorescence-labeled affinity peptide for Cholera Toxin B detection.
- Employing a model ON-OFF-ON system to study peptide sorption and desorption kinetics.
- Analyzing sorption and release kinetics using second-order kinetic models and considering π/hydrophobic interactions.
- Testing the system in bacterial cultures of *Vibrio cholerae* and *Vibrio parahaemolyticus*.
Main Results:
- Distinct surface properties of GDYO, GO, and BP significantly affected peptide loading/release capacity and kinetics.
- Sorption followed a second-order kinetic model, with chemisorption being dominant for BP micromotors.
- Faster release kinetics were observed for GDYO and GO micromotors, attributed to π and hydrophobic interactions.
- Micromotor movement enhanced performance in low sample volumes and high protein content environments.
- Successful illustration of loading/release capacity and sensing feasibility in bacterial cultures.
Conclusions:
- Janus micromotors functionalized with different 2D nanomaterials provide a versatile platform for peptide-based biosensing.
- Surface chemistry and micromotor dynamics are critical factors governing sensing performance.
- This novel approach shows significant promise for developing efficient micromotor-based sensors for biomedical applications.
More Related Videos
14:43Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
Published on: September 23, 2013
08:05Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces
Published on: September 9, 2022