Related Experiment Video

Updated: Aug 14, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
09:08

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Published on: January 13, 2017

Purification and characterization of cloned isopenicillin N synthetase

J E Baldwin, S J Killin, A J Pratt

    The Journal of Antibiotics
    |May 1, 1987
    PubMed

    More Related Videos

    Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli07:35

    Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli

    Published on: August 3, 2017

    Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids11:56

    Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

    Published on: May 4, 2018

    Related Concept Videos

    Production of Antibiotics01:27

    Production of Antibiotics

    Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
    Production of Pharmaceuticals01:30

    Production of Pharmaceuticals

    Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...