Related Experiment Video

Updated: Dec 6, 2025

Author Spotlight: Advancing Research on Candida albicans Biofilm-Associated Prosthetic Joint Infections
04:37

Author Spotlight: Advancing Research on Candida albicans Biofilm-Associated Prosthetic Joint Infections

Published on: February 2, 2024

1.1K

Time to positivity of acute and chronic periprosthetic joint infection cultures

D T Talsma1, J J W Ploegmakers2, P C Jutte2

  • 1Department of Medical Microbiology and Infection Prevention, University of Groningen, University Medical Center Groningen, Hanzeplein 1, 9700, RB, Groningen.

Diagnostic Microbiology and Infectious Disease
|October 5, 2020
PubMed
Abstract

Insights

Diagnosing acute periprosthetic joint infections (PJI) does not require prolonged incubation. Cultures from acute PJI grow quickly, unlike chronic PJI, and sonication offers no clear benefit.

Area of Science:

  • Orthopedic Surgery
  • Infectious Diseases
  • Medical Microbiology

Background:

  • Periprosthetic joint infection (PJI) diagnosis typically involves prolonged incubation times.
  • Current diagnostic protocols do not differentiate between acute and chronic PJI, potentially leading to unnecessary delays.

Purpose of the Study:

  • To evaluate the optimal incubation time for diagnosing acute versus chronic periprosthetic joint infections (PJI).
  • To assess the utility of prosthesis sonication in PJI diagnosis.

Main Methods:

  • Retrospective analysis of 59 patients with PJI undergoing surgical debridement.
  • Cultures of synovial fluid, periprosthetic tissue samples, and sonicated prosthesis.

Main Results:

  • Acute PJI isolates (21 patients) grew within 5 days, while chronic PJI isolates (38 patients) took 11 days.
  • Sonication fluid showed faster positivity for chronic PJI but not acute PJI compared to tissue cultures.

Conclusions:

  • Prolonged incubation is unnecessary for acute PJI diagnosis.
  • Prosthesis sonication provides no significant advantage in diagnosing acute PJI.
Keywords:
AcuteChronicDiagnosisPeriprosthetic joint infectionSonicationTime to positivity

More Related Videos

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction10:35

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Published on: December 3, 2017

11.4K
A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials06:18

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials

Published on: March 3, 2023

1.7K

Related Concept Videos

Acute Pyelonephritis II: Diagnostic Studies and Management01:28

Acute Pyelonephritis II: Diagnostic Studies and Management

Introduction:For diagnosing acute pyelonephritis, a comprehensive patient history is collected to identify symptoms such as dysuria, frequent or urgent urination, flank pain, or costovertebral angle (CVA) tenderness that may suggest a kidney infection.Physical ExaminationDuring the physical examination, CVA tenderness is assessed. This involves gentle percussion over the costovertebral angle, where tenderness often indicates a kidney infection.Diagnostic TestsUrinalysis: Used to identify white...
178
Acute Pyelonephritis I: Introduction01:27

Acute Pyelonephritis I: Introduction

Pyelonephritis is a bacterial infection that primarily affects the renal parenchyma and collecting system, including the renal pelvis, tubules, and interstitial tissue of one or both kidneys. It can be classified as either acute—a sudden, severe infection—or chronic, which refers to long-term or recurrent kidney infections.The primary cause of acute pyelonephritis (APN) is bacterial infection, with Escherichia coli accounting for approximately 70-80% of cases. Other bacteria, such...
298
Acute Pharyngitis01:30

Acute Pharyngitis

Introduction
Acute pharyngitis is the inflammation of the back of the throat (pharynx), commonly resulting in a sore throat. It is a frequently encountered condition that prompts individuals to seek medical advice.
Classification
Acute pharyngitis can be categorized based on its underlying cause:
3.0K
Factors Affecting the Risk of Infection01:26

Factors Affecting the Risk of Infection

The hosts' susceptibility to infection depends on several factors. The integrity of the skin and mucous membranes helps protect the body against microbial attacks. When the skin is altered, the chance of infection, limb loss, and even death increases.
The integrity and count of the white blood cells help the body resist pathogens and fight infection. When impaired, it reduces the body's resistance to pathogens. The acidic pH levels of the gastrointestinal, genitourinary tracts, and skin...
13.1K
Urine Studies II: Urine Culture and Sensitivity Test01:26

Urine Studies II: Urine Culture and Sensitivity Test

A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
460
Endocarditis I: Introduction01:25

Endocarditis I: Introduction

Introduction:Endocarditis is the infection of the endocardium, the inner lining of the heart and its valves. When the heart muscle is involved, the condition is termed myocarditis, while an infection of the outer lining is called pericarditis. Infective endocarditis (IE) primarily affects the endocardium, where pathogens adhere to the valves or lining, forming vegetation that can lead to severe complications. Infective endocarditis occurs when microorganisms, usually bacteria from other body...
254