A portable nucleic acid extraction system based on gigahertz acoustic tweezers

In this study, a portable and compatible system for extraction of DNA based on gigahertz acoustic was proposed and verified. The system is based on tunable multiple acoustic tweezers which can switch between the mixing and separation mode to enable the efficient DNA extraction with a relatively small and portable setup. Using this system, we extracted DNA from the rat's whole blood and achieved about 80% recovery. By combining with real-time qPCR, this proposed detection method was able to detect adenovirus (ADV) DNA as low as 10² IU/mL.Clinical Relevance-The whole system is only in centimeter scale and can be applied for portable DNA extraction. There is great potential application in molecular diagnostics and point of care test (POCT).