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Related Concept Videos

RNA Interference01:23

RNA Interference

27.4K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

747
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
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Types of RNA01:20

Types of RNA

8.5K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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Types of RNA01:23

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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Updated: Dec 6, 2025

Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches
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Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches

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Delivery Systems for RNA Interference Therapy: Current Technologies and Limitations.

Yi Wang1

  • 1School of Dentistry, The University of Queensland, Brisbane, Queensland 4006, Australia.

Current Gene Therapy
|October 6, 2020
PubMed
Summary
This summary is machine-generated.

RNA interference (RNAi) therapy faces challenges with agent stability and targeting. This review discusses current and novel drug delivery systems for RNAi agents, highlighting limitations and future potential.

Keywords:
RNA interferencealbuminexosomeliposomenucleic acid deliveryviral vectorxenobot

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Area of Science:

  • Biomedicine
  • Molecular Biology
  • Drug Delivery Systems

Background:

  • RNA interference (RNAi) technology shows therapeutic promise for various diseases by silencing target genes.
  • RNAi agents, typically nucleic acids, suffer from instability and lack of specificity in the circulatory system.
  • Effective drug delivery systems are essential to overcome these limitations and enable RNAi therapeutic applications.

Purpose of the Study:

  • To review the technical challenges in developing drug delivery systems for RNA interference agents.
  • To summarize current technologies used for delivering RNAi agents and discuss their inherent limitations.
  • To highlight emerging innovative drug delivery systems, such as configurable xenobots, for advancing RNAi therapeutics.

Main Methods:

  • Literature review of existing and emerging drug delivery systems for RNAi.
  • Analysis of technical difficulties associated with RNAi agent delivery.
  • Discussion of the limitations of current delivery technologies including viral, inorganic, polymeric, and lipid-based carriers.

Main Results:

  • Existing drug delivery systems (viral, inorganic, polymeric, lipid-based) have significant limitations for RNAi applications.
  • Novel systems like configurable xenobots represent a rapid advancement in the field.
  • Technical hurdles in stability and target specificity remain key challenges for RNAi delivery.

Conclusions:

  • Overcoming instability and targeting issues is critical for successful RNAi therapeutics.
  • Innovative drug delivery systems are crucial for realizing the full potential of RNAi technology.
  • Emerging technologies offer promising avenues for next-generation RNAi-based treatments.