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Published on: October 28, 2018
Design of spontaneously blinking fluorophores for live-cell super-resolution imaging based on quantum-chemical
Ryo Tachibana1, Mako Kamiya, Akihiko Morozumi
1Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. uranokun@m.u-tokyo.ac.jp.
Abstract:
Spontaneously blinking fluorophores are powerful tools for live-cell super-resolution imaging under physiological conditions. Here we show that quantum-chemical calculations can predict key parameters for fluorophore design. We applied this methodology to develop a spontaneously blinking fluorophore with yellow fluorescence for super-resolution imaging of microtubules in living cells.
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