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Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting

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Ultrasonic Microplotting of Microgel Bioinks.

D Chester^1,2,3, P Theetharappan^1,2, T Ngobili^1,2

¹Department of Biomedical Engineering, North Carolina State University, Raleigh, North Carolina 27695, United States.

ACS Applied Materials & Interfaces

|October 7, 2020

Summary

This summary is machine-generated.

Ultrasonic microplotting enables precise patterning of microgel films, overcoming limitations of layer-by-layer techniques for advanced biomaterial scaffolds.

Keywords:

bioink layer-by-layer fabrication microgel microplotting patterned films

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Published on: August 8, 2022

Area of Science:

Biomaterials Engineering
Tissue Engineering
Microfabrication

Background:

Material scaffolds mimicking native tissues are crucial for regenerative medicine.
Microgel particle scaffolds show potential in bone regeneration and cell growth.
Current layer-by-layer (LBL) techniques lack microscale deposition control for complex scaffolds.

Purpose of the Study:

To address limitations of LBL fabrication for microgel scaffolds.
To develop and optimize an ultrasonic microplotting technique for patterned microgel films.
To investigate printing parameters for high-resolution microgel film fabrication.

Main Methods:

Utilized ultrasonic microplotting for microgel film fabrication.
Optimized bioink formulation (2 mg/mL microgels, 20% polyethylene glycol), surface coating (bovine serum albumin), and print head diameter (50 μm).
Evaluated print quality and resolution based on optimized parameters.

Main Results:

Identified optimal printing parameters for high-quality microgel films.
Achieved patterned films with a maximum resolution of 50 μm.
Demonstrated ultrasonic microplotting's capability for complex microgel film creation.

Conclusions:

Ultrasonic microplotting offers superior control over microgel film fabrication compared to LBL.
This technique enables the creation of more complex microgel structures.
Potential for finer printing resolutions exists with further development, advancing biomaterial scaffold design.