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Ultrasonic Microplotting of Microgel Bioinks.

D Chester1,2,3, P Theetharappan1,2, T Ngobili1,2

  • 1Department of Biomedical Engineering, North Carolina State University, Raleigh, North Carolina 27695, United States.

ACS Applied Materials & Interfaces
|October 7, 2020
PubMed
Summary

Ultrasonic microplotting enables precise patterning of microgel films, overcoming limitations of layer-by-layer techniques for advanced biomaterial scaffolds.

Keywords:
bioinklayer-by-layer fabricationmicrogelmicroplottingpatterned films

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Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Microfabrication

Background:

  • Material scaffolds mimicking native tissues are crucial for regenerative medicine.
  • Microgel particle scaffolds show potential in bone regeneration and cell growth.
  • Current layer-by-layer (LBL) techniques lack microscale deposition control for complex scaffolds.

Purpose of the Study:

  • To address limitations of LBL fabrication for microgel scaffolds.
  • To develop and optimize an ultrasonic microplotting technique for patterned microgel films.
  • To investigate printing parameters for high-resolution microgel film fabrication.

Main Methods:

  • Utilized ultrasonic microplotting for microgel film fabrication.
  • Optimized bioink formulation (2 mg/mL microgels, 20% polyethylene glycol), surface coating (bovine serum albumin), and print head diameter (50 μm).
  • Evaluated print quality and resolution based on optimized parameters.

Main Results:

  • Identified optimal printing parameters for high-quality microgel films.
  • Achieved patterned films with a maximum resolution of 50 μm.
  • Demonstrated ultrasonic microplotting's capability for complex microgel film creation.

Conclusions:

  • Ultrasonic microplotting offers superior control over microgel film fabrication compared to LBL.
  • This technique enables the creation of more complex microgel structures.
  • Potential for finer printing resolutions exists with further development, advancing biomaterial scaffold design.