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Updated: Dec 6, 2025

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
Published on: September 2, 2021
CRISPRoff enables spatio-temporal control of CRISPR editing
Jared Carlson-Stevermer1, Reed Kelso2,3, Anastasia Kadina2
1Synthego Corporation, 3565 Haven Avenue, Menlo Park, CA, 94025, USA. jared.carlson-stevermer@synthego.com.
Abstract:
Following introduction of CRISPR-Cas9 components into a cell, genome editing occurs unabated until degradation of its component nucleic acids and proteins by cellular processes. This uncontrolled reaction can lead to unintended consequences including off-target editing and chromosomal translocations. To address this, we develop a method for light-induced degradation of sgRNA termed CRISPRoff. Here we show that light-induced inactivation of ribonucleoprotein attenuates genome editing within cells and allows for titratable levels of editing efficiency and spatial patterning via selective illumination.
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