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Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
Published on: January 9, 2019
Hsa-miR-27a-3p and epidermal growth factor receptor expression analysis in glioblastoma FFPE samples
Tayyebali Salmani1, Sayyed Mohammad Hossein Ghaderian1,2, Mohammadreza Hajiesmaeili3
1Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Aim:
Glioblastoma multiforme (GBM) is the most invasive type of glial tumors. MicroRNAs as the small, noncoding RNAs have been revealed that play an important role in tumorigenic processes. So, they may be used as potential biomarkers for detection and prognosis of cancers at the early stages. In addition, they can be applied as therapeutic targets. In the present study, the expression levels of hsa-miR-27a-3p and EGFR were investigated in GBM.
Methods:
Real-time RT-PCR was applied to evaluate hsa-miR-27a-3p and EGFR expressions in the formalin-fixed paraffin-embedded (FFPE) tissue samples obtained from 50 GBM and 50 normal people.
Results:
The expression level of hsa-miR-27a-3p and EGFR was significantly different between cases and controls. Positive association was found between gene expressions and immunohistochemistry markers, such as Ki67 and glial fibrillary acidic protein, except for IDH1 status.
Conclusion:
We showed the association of hsa-miR-27a-3p and EGFR with GBM and it can be concluded that they have a promising potential to use as primary cancer biomarkers.
Insights
This study investigated hsa-miR-27a-3p and EGFR in glioblastoma multiforme (GBM). Both were found to be significantly different in GBM patients, showing potential as cancer biomarkers.
Area of Science:
- Oncology
- Molecular Biology
- Biomarker Discovery
Background:
- Glioblastoma multiforme (GBM) is a highly aggressive brain tumor.
- MicroRNAs (miRNAs) are implicated in cancer development and progression.
- miRNAs show potential as early diagnostic and prognostic cancer biomarkers.
Purpose of the Study:
- To investigate the expression levels of hsa-miR-27a-3p and Epidermal Growth Factor Receptor (EGFR) in GBM.
- To assess the potential of hsa-miR-27a-3p and EGFR as biomarkers for GBM.
Main Methods:
- Real-time RT-PCR was used to quantify hsa-miR-27a-3p and EGFR expression.
- Formalin-fixed paraffin-embedded (FFPE) tissue samples from 50 GBM patients and 50 healthy controls were analyzed.
Main Results:
- Significant differences in hsa-miR-27a-3p and EGFR expression were observed between GBM patients and controls.
- A positive correlation was found between hsa-miR-27a-3p/EGFR expression and immunohistochemistry markers (Ki67, GFAP), excluding IDH1 status.
Conclusions:
- hsa-miR-27a-3p and EGFR are associated with Glioblastoma Multiforme.
- These molecules demonstrate promising potential as primary biomarkers for GBM detection and prognosis.
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