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Updated: Dec 6, 2025

Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue
Published on: October 6, 2022
Blue-light perception by epiphytic Pseudomonas syringae drives chemoreceptor expression, enabling efficient plant
Saray Santamaría-Hernando1, Jean Paul Cerna-Vargas1, Pedro Manuel Martínez-García1,2
1Centro de Biotecnología y Genómica de Plantas CBGP, Universidad Politécnica de Madrid-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Parque Científico y Tecnológico de la UPM Pozuelo de Alarcón, Madrid, Spain.
Abstract:
Adaptation and efficient colonization of the phyllosphere are essential processes for the switch to an epiphytic stage in foliar bacterial pathogens. Here, we explore the interplay among light perception and global transcriptomic alterations in epiphytic populations of the hemibiotrophic pathogen Pseudomonas syringae pv. tomato DC3000 (PsPto) following contact with tomato leaves. We found that blue-light perception by PsPto on leaf surfaces is required for optimal colonization. Blue light triggers the activation of metabolic activity and increases the transcript levels of five chemoreceptors through the function of light oxygen voltage and BphP1 photoreceptors. The inactivation of PSPTO_1008 and PSPTO_2526 chemoreceptors causes a reduction in virulence. Our results indicate that during PsPto interaction with tomato plants, light perception, chemotaxis, and virulence are highly interwoven processes.
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