Related Experiment Video
Updated: Dec 6, 2025

The Role of Indocyanine Green Fluorescence in Complex Laparoscopic Cholecystectomy Navigation
Published on: January 31, 2025
Indocyanine Green Fluorescence in Elective and Emergency Laparoscopic Cholecystectomy. A Visual Snapshot
Giovanni D Tebala1, Giles Bond-Smith1
1Surgical Emergency Unit, Department of General Surgery, John Radcliffe Hospital, Oxford University Hospitals NHS Foundation Trust, Oxford (UK).
Abstract:
Positive identification of the biliary anatomy is a crucial step during laparoscopic cholecystectomy to prevent iatrogenic biliary injuries. While it is usually quite straightforward in elective cholecystectomies, it may be very challenging in an emergency setting, when inflammation and adhesions at the gallbladder pedicle make identification of the common bile duct a difficult and risky manoeuvre. Indocyanine green (ICG) is a dye that, when injected intravenously at 0.2-0.5 mg/kg, concentrates in the bile and becomes fluorescent under near-infrared light. When administered well in advance, ideally 24 hours before the procedure, ICG is completely cleared by the liver and reaches a good concentration in the bile, thus allowing a good fluorescence-cholangiogram. Unfortunately, in emergency cholecystectomy-when it would be most needed-the injection of ICG cannot be planned with such long notice. However, even when injected less than 1 hour before the operation, ICG may be able to reach a sufficient concentration in the bile. This report shows that ICG-fluorescence can be helpful in identifying the extrahepatic biliary anatomy during the dissection of Calot's triangle in both elective and emergency cases.
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