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Improving sciatic nerve regeneration by using alginate/chitosan hydrogel containing berberine.

Majid Rahmati1, Arian Ehterami2, Reza Saberani3

  • 1Department of Medical Biotechnology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.

Drug Delivery and Translational Research
|October 9, 2020
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Summary

Alginate/chitosan hydrogels incorporating berberine show promise for peripheral nerve repair. The 1% berberine hydrogel significantly enhanced sciatic nerve regeneration in rats, offering a potential new treatment for nerve injuries.

Keywords:
Alginate/chitosanBerberineCrush injuryHydrogelSciatic nerveTissue engineering

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Neuroscience

Background:

  • Peripheral nerve injuries (PNIs) are common, leading to significant pain and disability.
  • Berberine possesses properties beneficial for neurological disorders and tissue repair.
  • Developing effective scaffolds is crucial for promoting nerve regeneration.

Purpose of the Study:

  • To create and evaluate alginate/chitosan (Alg/Chit) hydrogels containing varying concentrations of berberine as scaffolds for sciatic nerve regeneration.
  • To assess the physical, chemical, and biological properties of the berberine-loaded hydrogels.
  • To investigate the in vivo efficacy of these hydrogels in promoting peripheral nerve repair in a rat model.

Main Methods:

  • Alg/Chit hydrogels were synthesized and characterized for structure, swelling, weight loss, and pore size.
  • Berberine release kinetics, cytocompatibility (MTT assay), and hemocompatibility were evaluated.
  • Sciatic nerve crush injury was induced in rats, and hydrogels with different berberine concentrations were implanted.
  • Functional recovery was assessed to determine the therapeutic effect of the berberine-loaded hydrogels.

Main Results:

  • The hydrogels exhibited suitable physical properties, including an average pore size of 39 μm and significant weight loss over 21 days.
  • Cytocompatibility and hemocompatibility of the hydrogels were confirmed.
  • Berberine demonstrated a dose-dependent effect on cell proliferation.
  • In vivo studies showed that the hydrogel containing 1% berberine significantly promoted sciatic nerve regeneration and functional recovery.

Conclusions:

  • Alg/Chit hydrogel serves as a viable scaffold for peripheral nerve regeneration.
  • Berberine, particularly at a 1% concentration within the hydrogel, enhances nerve repair.
  • This berberine-loaded Alg/Chit hydrogel represents a promising therapeutic strategy for treating peripheral nerve injuries.