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Identification of An Optimized Collagen Extraction Protocol forRegenerative Medicine Application: A Comparative
Tayebeh Sadat Tabatabai1, Arian Ehterami2, Tahereh Sadat Tabatabai1
1Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
Cell Journal
|April 29, 2026
Summary
Protocol 4 is the best method for extracting high-quality collagen, preserving its structure and function for tissue engineering. This optimized collagen shows excellent biocompatibility and supports tissue regeneration in vivo.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Collagen is vital for tissue engineering and regenerative medicine due to its biocompatibility.
- Optimizing collagen extraction methods to maintain structural and functional integrity is crucial but challenging.
Purpose of the Study:
- To compare eleven collagen extraction protocols.
- To identify the most effective method for producing high-quality collagen.
Main Methods:
- Eleven collagen extraction protocols were evaluated.
- Characterization included SEM, FTIR, XRD, Raman spectroscopy, MTT, anti-inflammatory assays, LDH cytotoxicity, TAC, blood compatibility, protein quantification, SDS-PAGE, and solubility.
- In vivo evaluation involved subcutaneous transplantation in mice.
Main Results:
- Protocol 4 yielded the highest collagen (approx. 35 mg/g tissue) with preserved triple-helical structure and fiber morphology.
- Protocol 4 demonstrated high cell viability, low cytotoxicity, optimal blood compatibility, and significant antioxidant activity.
- In vivo studies showed normal tissue regeneration, including collagen deposition and angiogenesis, without inflammation.
Conclusions:
- Protocol 4 offers a robust method for extracting collagen with superior structural integrity, functional stability, and biocompatibility.
- Optimized collagen from Protocol 4 is suitable for biomedical applications and translational research.
