Upconversion optogenetic micro-nanosystem optically controls the secretion of light-responsive bacteria for systemic...

Area of Science:

• Microbiology
• Immunology
• Biotechnology

Background:

• Intestinal microbiota-derived molecules influence host immunity, offering a new therapeutic avenue for immune-related diseases.
• Effective regulation of engineered bacteria secretion remains a challenge for targeted therapies.

Purpose of the Study:

• To construct an upconversion optogenetic micro-nanosystem for precise regulation of engineered bacteria secretion.
• To demonstrate the system's efficacy in modulating immune responses for disease treatment.

Main Methods:

• Engineered bacteria were modified to be light-responsive for controlled secretion.
• An upconversion optogenetic micro-nanosystem was developed for optical sensing and manipulation.
• The system's ability to regulate secretion of immune factors (TGF-β1, IFN-γ) was tested in models of inflammatory bowel disease and tumors.

Main Results:

• Controllable secretion of transforming growth factor-beta 1 (TGF-β1) suppressed intestinal inflammation.
• Regulated secretion of interferon-gamma (IFN-γ) inhibited B16F10 tumor progression.
• The upconversion optogenetic system demonstrated effective optical control over engineered bacteria.

Conclusions:

• The developed upconversion optogenetic micro-nanosystem offers a novel strategy for regulating engineered bacteria secretion.
• This technology shows promise for developing targeted immunotherapies for inflammatory and oncological conditions.