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Published on: July 25, 2022
Magnetic Field Exposure Modulates the Anti-Inflammatory Efficiency of Minocycline in Rats with Peripheral Acute
Background And Objective:
Microglial activation in spinal cord is key contributor and its inhibition by Minocycline (MCN) can result in anti-inflammatory actions. Effect of pulsed magnetic field (PMF) in living system is a very complex process and many biological and cellular processes can play key roles. In this study aimed to reveal the roles of PMF exposure on anti-inflammatory potentials of MCN treatment by evaluating the inflammatory profiles of either inflamed site or spinal cord.
Methods:
In this study, we investigated the anti-inflammatory effects of PMF, MCN or their combination treatments in rats with carrageenan (CG)-induced peripheral inflammation by examining the cardinal signs, hyperalgesia, allodynia, edema and fever. The levels of various inflammation markers (tumor necrosis factor-α), interleukin (IL)-1β, IL-6, IL-17, IL-4, IL-10, C-C motif chemokine ligand3 (CCL3), C-X-C motif chemokine ligand1 and myeloperoxidase were also measured in paw and spinal cord tissues.
Results:
CG induced inflammation caused edema, fever, and hypersensitivities. MNC or PMF treatments ameliorated these responses by suppressing pro-inflammatory markers in both inflamed paw and spinal cord. Although anti-hypersensitive, anti-edematous and anti-pyretic actions of MCN or PMF, in combined treatments PMF exposure decreased the anti-hyperalgesic and anti-allodynic actions of MCN treatment. These may be associated with decreases in IL-4 and IL-10 levels and an increase in CCL3 level of spinal cord tissues.
Conclusion:
Present findings support that MCN or PMF has anti-inflammatory properties duo to the down-regulating central microglial and/or peripheral inflammatory markers. Our data showed here, for the first time, PMF exposure may suppress the anti-hypersensitive actions of MCN by modulating microglia function/phenotype and microglial markers.
Insights
Pulsed magnetic field (PMF) and Minocycline (MCN) show anti-inflammatory effects. However, PMF may reduce MCN
Area of Science:
- Neuroscience and Immunology
- Inflammation research
- Therapeutic modalities
Background:
- Microglial activation in the spinal cord is a significant contributor to inflammation.
- Minocycline (MCN) exhibits anti-inflammatory properties by inhibiting microglial activation.
- Pulsed magnetic field (PMF) effects on biological systems are complex and involve various cellular processes.
Purpose of the Study:
- To investigate the impact of PMF exposure on the anti-inflammatory efficacy of MCN.
- To evaluate the combined effects of PMF and MCN on inflammatory profiles in both peripheral and central nervous system sites.
- To elucidate the mechanisms underlying the interaction between PMF and MCN in managing inflammation.
Main Methods:
- Carrageenan (CG)-induced peripheral inflammation model in rats.
- Assessment of anti-inflammatory effects by measuring cardinal signs: hyperalgesia, allodynia, edema, and fever.
- Quantification of inflammatory markers including TNF-α, IL-1β, IL-6, IL-17, IL-4, IL-10, CCL3, CXCL1, and myeloperoxidase in paw and spinal cord tissues.
Main Results:
- Both MCN and PMF treatments individually reduced CG-induced inflammation, edema, fever, and hypersensitivities by suppressing pro-inflammatory markers.
- Combined PMF and MCN treatments showed a decrease in the anti-hyperalgesic and anti-allodynic effects of MCN.
- This suppression may be linked to reduced IL-4 and IL-10 levels and increased CCL3 levels in spinal cord tissues.
Conclusions:
- Both MCN and PMF possess anti-inflammatory properties by down-regulating central microglial and peripheral inflammatory markers.
- PMF exposure may attenuate the anti-hypersensitive actions of MCN.
- This interaction is potentially mediated by modulating microglia function, phenotype, and associated markers.
