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Updated: Dec 6, 2025

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

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Published on: February 16, 2022

MeCas12a, a Highly Sensitive and Specific System for COVID-19 Detection.

Peixiang Ma¹, Qingzhou Meng², Baoqing Sun³

¹Shanghai Institute for Advanced Immunochemical Studies ShanghaiTech University Shanghai 201210 China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|October 12, 2020

Summary

Manganese-enhanced Cas12a (MeCas12a) significantly boosts nucleic acid detection sensitivity and specificity for infectious diseases. This advanced diagnostic tool can identify as few as five RNA copies and differentiate between similar viruses like SARS-CoV-2 and MERS-CoV.

Keywords:

COVID‐19 CRISPR/Cas12a CRISPR‐based detection SARS‐CoV‐2 manganese

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Area of Science:

Molecular Biology
Biotechnology
Infectious Disease Diagnostics

Background:

Cas12a systems offer rapid nucleic acid detection through collateral DNA cleavage.
Current diagnostic methods face limitations in sensitivity and specificity for certain infectious agents.

Purpose of the Study:

To develop a highly sensitive and specific Cas12a-based diagnostic system.
To enhance the detection capabilities of Cas12a for infectious disease diagnosis, including coronaviruses.

Main Methods:

Development of Manganese-enhanced Cas12a (MeCas12a) system.
Utilizing manganese to amplify signal and increase detection sensitivity.
Testing MeCas12a for detection of target RNAs and differentiation of single nucleotide polymorphisms (SNPs).

Main Results:

MeCas12a achieved up to a 13-fold increase in detection sensitivity.
The system successfully detected as few as five copies of target RNA.
MeCas12a demonstrated high specificity, distinguishing single nucleotide polymorphisms and differentiating between SARS-CoV-2 and MERS-CoV RNA.

Conclusions:

Manganese enhancement significantly improves Cas12a-based nucleic acid detection.
MeCas12a offers a highly sensitive and specific platform for diagnosing infectious diseases like COVID-19 and MERS.
This technology presents a promising alternative for rapid and accurate infectious disease diagnostics.