Related Experiment Video
Updated: Dec 6, 2025
08:07
Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques
Published on: January 12, 2024
1.1K
Highly sensitive serological approaches for Pepino mosaic virus detection
Wan-Qin He1, Jia-Yu Wu2, Yi-Yi Ren2
1State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
Journal of Zhejiang University. Science. B
|October 12, 2020
Summary
Researchers developed new monoclonal antibodies (mAbs) and three ELISA methods to detect Pepino mosaic virus (PepMV) in tomato plants. These tools offer sensitive and reliable detection for managing this widespread viral disease.
Area of Science:
- Plant Pathology
- Virology
- Immunotechnology
Background:
- Pepino mosaic virus (PepMV) poses a significant threat to global tomato production.
- Current viral detection methods lack the sensitivity and high-throughput capabilities needed for effective management.
- Developing novel diagnostic tools is crucial for controlling PepMV outbreaks.
Purpose of the Study:
- To generate specific monoclonal antibodies (mAbs) against PepMV.
- To establish and validate sensitive, high-throughput diagnostic assays for PepMV detection.
- To assess the efficacy of these methods for field monitoring of PepMV in tomato plants.
Main Methods:
- Purification of PepMV virions from infected Nicotiana benthamiana.
- Production of anti-PepMV mAbs in BALB/c mice using hybridoma technology.
- Development and optimization of Double Antibody Sandwich ELISA (DAS-ELISA), Dot-ELISA, and Tissue print-ELISA.
- Validation of serological methods against RT-PCR and DNA sequencing.
Main Results:
- A panel of six specific and sensitive anti-PepMV mAbs was successfully produced.
- DAS-ELISA and Dot-ELISA demonstrated high sensitivity in detecting PepMV in crude plant extracts.
- Tissue print-ELISA emerged as the most practical method for on-site detection.
- All developed serological assays were validated and showed reliable performance comparable to molecular methods.
Conclusions:
- The developed anti-PepMV mAbs are valuable tools for diagnostic assay development.
- The established DAS-ELISA, Dot-ELISA, and Tissue print-ELISA provide effective and reliable methods for PepMV detection.
- These novel diagnostic approaches will aid in the epidemiological study and management of PepMV in tomato crops, particularly in regions like Yunnan Province, China.
