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Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
Published on: August 15, 2019
Microbial physiology and interactions in anammox systems with the intermittent addition of organic carbons
Tianqi Zhang1, Qidong Yin1, Yunhong Shi2
1Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, Guangdong, China.
Abstract:
Organic carbon can affect nitrogen removal in the anaerobic ammonia oxidation (anammox) process. Two continuous up-flow anaerobic sludge blanket (UASB) reactors were operated under autotrophic (UASBN, without organic carbon) and mixotrophic (UASBCN, with the intermittent addition of acetate and propionate) conditions. Stable operation of anammox systems was achieved, with the nitrogen removal rate and percentage of 2.12 g/(L·d) and 86.4% in UASBN, and 2.09 g/(L·d) and 85.0% in UASBCN, respectively. The network of Candidatus Kuenenia, Thauera, and Nitrosomanas contributed to both nitrogen and carbon metabolisms, and the intermittent addition of acetate and propionate strengthened Ca. Kuenenia's ability to utilize several types of carbon sources. Anammox bacteria showed activity in the presence of organic carbon and without inorganic carbon, confirming the mixotrophic characteristic of Ca. Kuenenia. Cross-feeding of amino acids and vitamins existed among functional microorganisms, with extracellular polymeric substances acting as the media for microbial interactions.
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