Related Experiment Video
Updated: Dec 6, 2025
09:51
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
35.1K
dbGuide: a database of functionally validated guide RNAs for genome editing in human and mouse cells
Alexander A Gooden1, Christine N Evans1, Timothy P Sheets1
1Laboratory Animal Sciences Program, Frederick National Lab for Cancer Research, Frederick, MD 21702, USA.
Nucleic Acids Research
|October 14, 2020
Summary
dbGuide is a new database featuring over 4000 functionally validated guide RNA sequences for CRISPR/Cas9 gene editing in human and mouse. This resource aids researchers by providing experimentally confirmed sequences, improving CRISPR experiment success rates.
Area of Science:
- Molecular Biology
- Genetics
- Bioinformatics
Background:
- CRISPR technology is widely used for genetic perturbations across various organisms.
- Numerous computational tools exist for designing guide RNAs, but experimental validation is crucial.
- A need exists for a centralized resource of validated guide RNA sequences.
Purpose of the Study:
- To present dbGuide, a comprehensive database of functionally validated guide RNA sequences for CRISPR/Cas9-based knockout.
- To provide researchers with reliable, experimentally confirmed sequences to enhance CRISPR experiment design and success.
Main Methods:
- Compilation of computationally designed guide RNA sequences.
- Inclusion of over 4000 functionally validated sequences through direct amplicon sequencing.
- Manual curation of validated sequences from over 1000 scientific publications.
Main Results:
- dbGuide offers a valuable resource of validated guide RNA sequences for human and mouse CRISPR/Cas9 knockout.
- The database integrates both predicted and experimentally verified sequences.
- The framework supports continuous updates with new data and expansion to other gene editing systems.
Conclusions:
- dbGuide significantly aids researchers by providing validated CRISPR guide RNA sequences, reducing trial-and-error.
- The database serves as a valuable tool for advancing CRISPR-based research in human and mouse models.
- Future development will incorporate additional gene editing systems, species, and functionalities like base editing and epigenetic modification.
