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Engineering and Application of a Myoglobin Binding Split Aptamer
Shaoyuan Li1, Yan Zheng1, Qingqing Zou1
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, P. R. China.
Analytical Chemistry
|October 14, 2020
Summary
This study introduces a reliable method for engineering split aptamers, enhancing biosensing capabilities. The developed split aptamers successfully detect cardiac biomarker myoglobin (Myo) in human serum.
Area of Science:
- Biomedical Engineering
- Biosensing Technology
- Molecular Diagnostics
Background:
- Split aptamers are crucial for developing sandwich assays when only one aptamer is available.
- Existing methods for engineering split aptamers that bind proteins are limited by a lack of understanding of binding mechanisms and reliable techniques.
Purpose of the Study:
- To explore a novel strategy for engineering split aptamers targeting proteins.
- To develop a reliable method for detecting the cardiac biomarker myoglobin (Myo) using engineered split aptamers.
Main Methods:
- Utilized atomic force spectroscopy (AFM) to engineer split aptamers by optimizing binding probability.
- Employed dynamic light scattering (DLS) for the detection of Myo using the engineered split aptamers.
Main Results:
- Successfully engineered split aptamers capable of binding to the target protein, myoglobin.
- Demonstrated the utility of these split aptamers for detecting Myo in complex biological matrices like human serum.
Conclusions:
- The proposed strategy for engineering split aptamers is intuitive, reliable, and offers a general approach for obtaining split aptamers against protein targets.
- This work advances biosensing by providing a robust method for split aptamer development and Myo detection.
