Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Screens02:46

Genetic Screens

5.4K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.4K
CRISPR01:59

CRISPR

56.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
56.0K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.2K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondria limit coenzyme Q export under cholesterol biosynthetic stress.

The Journal of cell biology·2026
Same author

Correction to: Elevated APOBEC3B expression drives a kataegic-like mutation signature and replication stress-related therapeutic vulnerabilities in p53-defective cells.

British journal of cancer·2026
Same author

Publisher Correction: Toxoplasma effector TgROP1 establishes membrane contact sites with the endoplasmic reticulum during infection.

Nature microbiology·2025
Same author

Toxoplasma effector TgROP1 establishes membrane contact sites with the endoplasmic reticulum during infection.

Nature microbiology·2025
Same author

ERK pathway agonism for cancer therapy: evidence, insights, and a target discovery framework.

NPJ precision oncology·2024
Same author

Practical Characterization Strategies for Comparison, Qualification, and Selection of Cell Viability Detection Methods for Cellular Therapeutic Product Development and Manufacturing.

Journal of fluorescence·2023

Related Experiment Video

Updated: Dec 5, 2025

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

22.8K

A Flexible, Pooled CRISPR Library for Drug Development Screens.

Maximilian Blanck1, Milka B Budnik-Zawilska1, Steven R Lenger2

  • 1Horizon Discovery, Cambridge, United Kingdom.

The CRISPR Journal
|October 15, 2020
PubMed
Summary

A new, modular CRISPR screening library improves negative selection screens for drug discovery. This scalable library enhances target identification and validation across the drug development pipeline.

More Related Videos

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.6K
Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

870

Related Experiment Videos

Last Updated: Dec 5, 2025

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

22.8K
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.6K
Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

870

Area of Science:

  • Genomics
  • Molecular Biology
  • Drug Discovery

Background:

  • CRISPR screening enables high-throughput gene manipulation analysis.
  • Negative selection screening is crucial but challenging in drug discovery.

Purpose of the Study:

  • To assess a new modular genome-wide CRISPR library for improved negative selection screening.
  • To evaluate its utility throughout the drug development pipeline.

Main Methods:

  • Developed a scalable, modular CRISPR library split into three parts.
  • Tested the library using a poly(ADP-ribose) polymerase 1 (PARP1) inhibitor to identify sensitizing genes.
  • Simulated hit identification and validated findings via orthogonal drug combination cell panel screening.

Main Results:

  • The modular library demonstrated improved negative selection screening capabilities.
  • Identified genes that sensitize cells to the PARP1 inhibitor.
  • Validated hits using high-throughput cell panel screens.

Conclusions:

  • A scalable CRISPR knockout screening library offers multiple applications in drug development.
  • Initial hit validation is achievable through high-throughput cell panel screens.