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Updated: Dec 5, 2025

Analysis of Effect of Compound Salt Stress on Seed Germination and Salt Tolerance Analysis of Pepper Capsicum annuum L.
Published on: November 30, 2022
Cotton seedling drought tolerance is improved via salt preconditioning
Rong Fan1, Xiujuan Su1, Yaping Guo1
1College of Agronomy, Xinjiang Agricultural University, Ürümqi, Xinjiang, China.
Adding NaCl to PEG stress partially alleviates drought damage in cotton seedlings. Three transcription factors (GhHsfA, GhbZIP, GhNAC) serve as molecular indicators for drought resistance, with ND359-5 identified as the most resistant variety.
Area of Science:
- Plant Physiology
- Molecular Biology
- Agricultural Science
Background:
- Drought stress significantly impacts cotton yield and quality.
- Understanding cotton's response to combined stresses like drought and salinity is crucial for crop improvement.
Purpose of the Study:
- To investigate the effects of PEG and NaCl stress, individually and combined, on cotton seedling physiology.
- To identify molecular markers for assessing drought resistance in cotton.
- To evaluate the protective role of NaCl pretreatment against PEG-induced drought stress.
Main Methods:
- Upland cotton seedlings subjected to PEG, NaCl, and combined NaCl/PEG stress treatments.
- Measurement of physiological indicators: malondialdehyde (MDA), proline (Pro), superoxide dismutase (SOD), peroxidase (POD), and relative electrolyte leakage (REL).
- Analysis of stress-related transcription factors (GhHsfA, GhbZIP, GhNAC) gene expression and correlation with physiological data.
Main Results:
- Combined NaCl/PEG stress induced higher SOD and POD activity but lower MDA content and REL compared to PEG alone.
- Cluster analysis indicated damage severity: PEG > NaCl > NaCl/PEG, suggesting NaCl mitigates PEG's adverse effects.
- Transcription factor expression levels strongly correlated with physiological responses, identifying them as key molecular indicators.
Conclusions:
- Appropriate NaCl levels can partially alleviate drought stress in cotton.
- GhHsfA, GhbZIP, and GhNAC are reliable molecular indicators for cotton drought resistance.
- Variety ND359-5 demonstrated superior drought resistance, offering valuable genetic resources for breeding programs.
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