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MiR-506-3p suppresses papillary thyroid cancer cells tumorigenesis by targeting YAP1

Lei Chen1, Xuehui Wang1, Changle Ji2

  • 1Clinical Medical College of Shanghai Tenth People's Hospital, Nanjing Medical University, Nanjing 211166, China; Department of Thyroid and Breast Surgery, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, China.

Pathology, Research and Practice
|October 15, 2020
PubMed

Insights

MicroRNA miR-506-3p inhibits papillary thyroid cancer (PTC) cell growth by targeting YAP1. This microRNA suppresses proliferation and disrupts the cell cycle, offering potential therapeutic strategies for thyroid cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Thyroid cancer (TC) is the most prevalent endocrine malignancy globally, with papillary thyroid cancer (PTC) accounting for 80-85% of cases.
  • MicroRNAs (miRNAs) are key regulators of gene expression, influencing tumorigenesis and cancer development.

Purpose of the Study:

  • To investigate the functional role and molecular mechanisms of miR-506-3p in papillary thyroid cancer.

Main Methods:

  • In vitro experiments were conducted to assess cell proliferation and cell cycle.
  • Western Blot and RT-qPCR were used to analyze gene and protein expression levels.
  • The direct targeting of YAP1 by miR-506-3p was investigated.

Main Results:

  • miR-506-3p significantly suppressed PTC cell proliferation and disrupted the cell cycle in vitro.
  • YAP1 was identified as a direct target gene of miR-506-3p.
  • miR-506-3p down-regulated YAP1 expression at both mRNA and protein levels, consequently affecting CDK2/Cyclin E1 expression.

Conclusions:

  • miR-506-3p exhibits tumor-suppressive functions in PTC by inhibiting YAP1 expression.
  • The miR-506-3p/YAP1 axis regulates the YAP1-CDK2/Cyclin E1 pathway, impacting cell cycle progression in PTC.
Keywords:
Papillary thyroid cancerProliferationYAP1miR-506-3p

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