Related Experiment Video
Updated: Dec 5, 2025
09:33
Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
Published on: February 7, 2018
7.7K
H2O2/HOCl-based fluorescent probes for dynamically monitoring pathophysiological processes
1Shaanxi Province Key Laboratory of Catalytic Foundation and Application, College of Chemistry and Environment Science, Shaanxi University of Technology, Hanzhong 723001, China. zhangdan@snut.edu.cn.
The Analyst
|October 16, 2020
Summary
This review explores fluorescent probes for detecting hydrogen peroxide (H2O2) and hypochlorous acid (HOCl). These probes are vital for diagnosing diseases linked to ROS imbalance and guiding medical treatments.
Area of Science:
- Biochemistry
- Medical Diagnostics
- Chemical Biology
Background:
- Reactive oxygen species (ROS), including hydrogen peroxide (H2O2) and hypochlorous acid (HOCl), are critical in biological redox balance.
- Imbalances in H2O2/HOCl levels are implicated in diseases like inflammation, cancer, and neurodegeneration.
- Accurate regulation and monitoring of H2O2/HOCl are essential for pathological diagnosis and effective therapy.
Purpose of the Study:
- To review the optical properties of fluorescent probes responsive to H2O2 and HOCl.
- To focus on the distribution and dynamic monitoring capabilities of these probes in pathophysiological models.
Main Methods:
- Literature review of H2O2/HOCl-responsive fluorescent probes.
- Analysis of probe optical properties.
- Evaluation of probe distribution and dynamic monitoring in disease models.
Main Results:
- Fluorescent probes offer high spatial and temporal resolution for ROS detection.
- Recent advancements have rapidly improved probe development over the last five years.
- Effective distribution and dynamic monitoring strategies are key for clinical application.
Conclusions:
- H2O2/HOCl-responsive fluorescent probes are powerful tools for understanding and diagnosing ROS-related diseases.
- Further development and application in pathophysiological models will advance medical diagnostics and therapeutics.
Related Concept Videos
01:19Protein Dynamics in Living Cells
2.5K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.5K
03:31Labeling DNA Probes
9.0K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.0K